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Cells were then incubated with 10 l CCK-8 solution for 1 h at 37 C, 5%CO 2

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Signs of Correct Administration A successful B12 injection should cause: Minimal pain at the site A small bump that fades within an hour Mild redness or soreness that resolves within 24 hours If symptoms worsen (swelling, pain, fever, rash), consult a doctor immediately